CRISPR activation
Enlarge text Shrink text-
Topic
-
Save successfulThe item can be found in your Personal ZoneשגיאהLog in to your account to save
Information for Authority record
Other Identifiers
- Work cat.: Dong, C. Programming bacterial gene expression using synthetic CRISPR-Cas transcriptional regulators, 2019:abstr. (The CRISPR-Cas activation (CRISPRa) system, a programmable transcriptional activator with wide applications in eukaryotic organisms, has been under-utilized in bacteria due to the lack of efficient transcriptional activation domains. This work describes our contribution to the development and understanding of bacterial CRISPR-Cas-based transcriptional regulation devices; the CRISPRa system; this work provided a novel programmable gene activation device in bacteria)
- CRISPRa and CRISPRi : gene expression modulation, via OriGene website, Aug. 7, 2020(CRISPR/Cas9 has recently adapted to generate two new technologies that modulates gene expression: CRISPRa (CRISPR activation) for gene activation and CRISPRi (CRISPR interference) for gene expression interference. In both CRISPRa and CRISPRi systems, the enzymatically deficient Cas9 (dCas9) is fused or interacts with transcriptional effector(s))
- What is CRISPRa vs CRISPRi?, via Horizon website, Aug. 7, 2020(The CRISPR-Cas9 system has also been adapted to generate technologies called CRISPRi (CRISPR interference) and CRISPRa (CRISPR activation). These utilize nuclease-deactivated Cas9 (dCas9) that cannot generate a DSB [DNA double-stranded break], but instead target genomic regions resulting in RNA-directed transcriptional control. CRISPRi utilizes dCas9 with or without a KRAB effector domain that complexes with gRNA to target promoter regions for transcriptional repression, or knockdown, of the gene. CRISPRa employs dCas9 fused to different transcriptional activation domains, which can be directed to promoter regions by either standard S. pyogenes gRNA or special gRNAs that recruit additional transcriptional activation domains to upregulate expression of the target gene)
- Kampmann, M. CRISPRi and CRISPRa : new functional genomics tools provide complementary insights into cancer biology and therapeutic strategies, 2018, via National Cancer Institute Office of Cancer Genomics website, Aug. 7, 2020(the genetic engineering system c̲lustered r̲egularly i̲nterspaced s̲hort p̲alindromic r̲epeats/C̲RISPR-a̲s̲sociated protein 9 (CRISPR/Cas9); the CRISPR/Cas9 system was first discovered in bacteria, which use it as an immune mechanism to degrade viral and plasmid DNA; When dCas9 is fused to the SunTag, a sequence containing multiple copies of the activator recruitment domain of general control protein (GCN4), the system activates transcription and is referred to as CRISPR activation (CRISPRa))
Wikipedia description:
CRISPR activation (CRISPRa) is a genetic perturbation technique that utilizes an engineered form of the CRISPR-Cas9 system to enhance the expression of specific genes without altering the underlying DNA sequence. Unlike traditional CRISPR-Cas9, which introduces double-strand breaks to edit genes, CRISPRa employs a modified, catalytically inactive Cas9 (dCas9) fused with transcriptional activators to target promoter or enhancer regions, thereby boosting gene transcription. This method allows for precise control of gene expression, making it a valuable tool for studying gene function, creating gene regulatory networks, and developing potential therapeutic interventions for a variety of diseases. Like for CRISPR interference, the CRISPR effector is guided to the target by a complementary guide RNA. However, CRISPR activation systems are fused to transcriptional activators to increase expression of genes of interest. Such systems are usable for many purposes including but not limited to, genetic screens and overexpression of proteins of interest. The most commonly used effectors are based on Cas9 (from Type II systems), but effectors based on other CRISPR-associated proteins, such as Cas12a (Type V), have been engineered as well.
Read more on Wikipedia >